14 Substance Testing Proposals Under REACH Registration Set for Approval

On October 20, 2025, the European Chemicals Agency (ECHA) announced the addition of 14 new substance testing proposals and invited public comments by December 4. ECHA’s public consultation on testing proposals is part of an open evaluation process to determine whether additional testing is required for registered chemicals.

Under the REACH Regulation, before conducting tests listed in Annex IX and X (corresponding to registration tonnage bands of 100–1,000 tonnes per year and over 1,000 tonnes per year), registrants must submit a testing proposal (TP). After the consultation period, ECHA will finalize the testing requirements based on feedback and the specific properties of the substances.

By gathering public input, ECHA aims to optimize safety assessments and revise testing requirements to align with stakeholder feedback. Registrants are obligated to carry out these tests and update their safety dossiers to ensure the safe use of chemicals and promote sustainable development in the industry.

Name	EC Number	CAS Number	Deadline for submitting information	Hazard endpoint for which vertebrate testing was proposed
Fatty acids, C16-18 and C18-unsatd., epoxidized, Me esters, oligomeric reaction products with trimethylolpropane	701-433-4	-	04/12/2025	6.1.2 Long-term toxicity to fish
Fatty acids, C16-C18 and C18-unsatd., ME esters, epoxidized	605-143-8	158318-67-3	04/12/2025	6.1.2 Long-term toxicity to fish
N,N'-bis(3-aminopropyl)ethylenediamine	234-147-9	10563-26-5	04/12/2025	7.6.2 Genetic toxicity in vivo
Sodium acetate	204-823-8	127-09-3	04/12/2025	6.1.2 Long-term toxicity to fish
Soybean oil, epoxidized, reaction products with methanol	287-837-7	85586-35-2	04/12/2025	6.1.2 Long-term toxicity to fish\|\|Note: testing proposed with Fatty acids, C16-18 and C18-unsatd., 2-ethylhexyl esters, epoxidized (EC no. 701-432-9)
tert-butyl acetate	208-760-7	540-88-5	04/12/2025	6.1.2 Long-term toxicity to fish
Zinc di(acetate)	209-170-2	557-34-6	04/12/2025	6.1.2 Long-term toxicity to fish
Fatty acids, C16-18 (even numbered, C18 unsaturated), 2-ethylhexyl esters, epoxidized	701-432-9	-	04/12/2025 04/12/2025	6.1.2 Long-term toxicity to fish 6.1.2 Long-term toxicity to fish \|\|Note: testing proposed with Fatty acids, C16-C18 and C18-unsatd., ME esters, epoxidized (EC no. 605-143-8, CAS no. 158318-67-3)
Fatty acids, C16-18 and C18-unsatd., Me esters, epoxidized, reaction products with ethylene glycol	606-712-3	211450-54-3	04/12/2025 04/12/2025	6.1.2 Long-term toxicity to fish\|\|Note: testing proposed with Fatty acids, C16-18 and C18-unsatd., 2-ethylhexyl esters, epoxidized (EC no. 701-432-9) 7.5.1 Repeated dose toxicity: oral\|\|Note: testing proposed with Soybean oil, epoxidized, reaction products with methanol (EC no. 287-837-7)
By-product from Guanidinoacetic acid manufacturing	951-773-4	-	04/12/2025 04/12/2025 04/12/2025	6.1.2 Long-term toxicity to fish 7.5.1 Repeated dose toxicity: oral 7.8.2 Developmental toxicity / teratogenicity

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